APMAdvances in Precision Medicine2424-85922424-9106Bio-Byword Scientific Publishing Pty. Ltd.10.26689/APM.v11i7.15576ArticleLong non-coding RNA LINC01123 facilitates the progression of lung cancer by targeting miR-384https://artdesignp.com/journal/APM/11/7/10.26689/APM.v11i7.15576ZhangYa,CaoGang20261172026-07-26Purpose: Although growing evidence has demonstrated that LINC01123 plays an oncogenic role in lung cancer, the underlying mechanisms require further elucidation. Methods: To explore the functions of LINC01123 on cell proliferation and migration, CCK-8 and Transwell assays were performed. The relationships between LINC01123 and miR-384 were examined by luciferase reporter assays. The expression of LINC01123 and miR-384 was quantified by quantitative real-time PCR. Results: In this study, knockdown of LINC01123 inhibited cell proliferation and migration, while overexpression of LINC01123 enhanced these effects in lung cancer. Luciferase reporter assays verified that LINC01123 could bind directly to miR-384. Overexpression of miR-384 suppresses cell proliferation and migration. The results of the rescue experiment showed that knockdown of LINC01123 inhibited cell proliferation and migration, whereas inhibition of miR-384 counteracted the repressive effects induced by LINC01123 inhibition. Conclusion: Our study reveals that LINC01123 promotes lung cancer progression via miR-384 for the first time.Lung cancer,LINC01123,miR-384

[1] Thai A, Solomon B, Sequist L, et al., 2021, Lung Cancer. Lancet, 398(10299): 535–554. [2] Hirsch F, Scagliotti G, Mulshine J, et al., 2017, Lung Cancer: Current Therapies and New Targeted Treatments. Lancet, 389(10066): 299–311. [3] Ning J, Ge T, Jiang M, et al., 2021, Early Diagnosis of Lung Cancer: Which Is the Optimal Choice? Aging (Albany NY), 13(4): 6214–6227. [4] Nooreldeen R, Bach H, 2021, Current and Future Development in Lung Cancer Diagnosis. International Journal of Molecular Sciences, 22(16): 8661. [5] Liu C, Xiang X, Han S, et al., 2022, Blood-Based Liquid Biopsy: Insights into Early Detection and Clinical Management of Lung Cancer. Cancer Letters, 524: 91–102. [6] Djebali S, Davis C, Merkel A, et al., 2012, Landscape of Transcription in Human Cells. Nature, 489(7414): 101–108. [7] Hangauer M, Vaughn I, McManus M, 2013, Pervasive Transcription of the Human Genome Produces Thousands of Previously Unidentified Long Intergenic Noncoding RNAs. PLoS Genetics, 9(6): e1003569. [8] Kung J, Colognori D, Lee J, 2013, Long Noncoding RNAs: Past, Present, and Future. Genetics, 193(3): 651–669. [9] Schmitz S, Grote P, Herrmann B, 2016, Mechanisms of Long Noncoding RNA Function in Development and Disease. Cellular and Molecular Life Sciences, 73(13): 2491–2509. [10] Shigeyasu K, Toden S, Ozawa T, et al., 2020, The PVT1 lncRNA Is a Novel Epigenetic Enhancer of MYC, and a Promising Risk-Stratification Biomarker in Colorectal Cancer. Molecular Cancer, 19(1): 155. [11] Lin S, Gregory R, 2015, MicroRNA Biogenesis Pathways in Cancer. Nature Reviews Cancer, 15(6): 321–333. [12] Cai F, Chen L, Sun Y, et al., 2020, MiR-539 Inhibits the Malignant Behavior of Breast Cancer Cells by Targeting SP1. Biochemistry and Cell Biology, 98(3): 426–433. [13] Zhao Y, Zhang J, Yang J, et al., 2022, MiR-205-5p Promotes Lung Cancer Progression and Is Valuable for the Diagnosis of Lung Cancer. Thoracic Cancer, 13(6): 832–843. [14] Wang Y, Zhu H, Zhang Z, et al., 2018, MiR-384 Inhibits the Proliferation of Colorectal Cancer by Targeting AKT3. Cancer Cell International, 18: 124. [15] Yan L, Wu K, Du F, et al., 2019, MiR-384 Suppressed Renal Cell Carcinoma Cell Proliferation and Migration Through Targeting RAB23. Journal of Cellular Biochemistry, 120(2): 1420–1426.