BASBone and Arthrosurgery Science3083-48562981-8222Bio-Byword Scientific Publishing Pty. Ltd.10.26689/bas.v3i4.11744ArticleResearch on Serum Biomarkers in Knee Joint Diseases of the Elderlyhttps://artdesignp.com/journal/BAS/3/4/10.26689/bas.v3i4.11744GuanJikui,ZhaoLi2025342025-09-04Background: Knee osteoarthritis (KOA) is characterized by chronic degeneration or wear of the articular cartilage, cartilage degeneration, fragmentation, and hardening, as well as bone spur formation and synovial inflammation. Over time, these changes occur slowly, and bone wear becomes more severe. This in turn causes pain, stiffness and swelling. It has an impact on normal work and life. To understand the mechanism and monitoring index of cartilage injury in the elderly population and prevent osteoarthritis. The purpose of this study was to compare the changes of serum cytokine biomarker levels in elderly patients with knee injury and osteoarthritis, and explore the correlation with the severity of lesions, which is conducive to early diagnosis and prevention of knee diseases. Objective: To investigate the differences in serum concentrations of matrix metalloproteinase-3 (MMP-3) and cartilage oligomeric matrix protein (COMP) in elderly patients with osteoarthritis, knee fracture or ligament injury. Methods: A total of 36 elderly KOA patients who underwent knee replacement in our hospital from July 2021 to May 2023 were selected as the observation group, and 36 elderly patients with knee fractures, patellar fractures, ligament or meniscus injuries who were treated during the same period were selected as the control group for the study. The age, gender, weight and other data of the patients were compared. The anteroposterior and lateral radiographs of the knee joints were examined for the first time, and the Kellgren and Lawrence (KL) score was used to compare the severity of KOA. The levels of serum MMP-3 and COMP in the two groups of patients were tested by enzyme-linked immunosorbent assay, and the changes and sensitivity of the two biomarkers in elderly knee diseases were compared and analyzed. Results: (1) Among the 72 knee joints included in the study, 36 cases were assigned to each group. No significant differences were observed in gender, age, or weight between the two groups (P > 0.05). (2) The concentrations of serum MMP-3 and COMP in the observation group were significantly higher than those in the control group. Conclusion:The elevated levels of COMP and MMP-3 in patients with knee osteoarthritis (KOA) suggest a potential correlation with disease progression, as their concentrations increased with worsening severity. These biomarkers may hold significant value for early detection, diagnosis, and prevention of KOA in elderly populations, warranting further investigation.

Berenbaum J, 2013, Osteoarthritis as an Inflammatory Disease (Osteoarthritis Is Not Osteoarthritis!). Osteoarthritis Chondroitin, 21: 16–21.

Mobasheri A, Bay-Jensen A, Spil W, et al., 2017, Osteoarthritis Year in Review 2016: Biomarkers (Biochemical Markers). Osteoarthritis Cartilage, 25(2): 199–208.

Glyn-Jones S, Palmer A, Agricola R, et al., 2015, Osteoarthritis. Lancet, 386(9991): 376–387.

Joint Surgery Group of Chinese Orthopaedic Association, 2018, Guidelines for the Diagnosis and Treatment of Osteoarthritis (2018 Edition). Chinese Journal of Orthopaedics, 38(12): 705–715.

Geurts J, Jurić D, Müller M, et al., 2018, Novel Ex Vivo Human Osteochondral Explant Model of Knee and Spine Osteoarthritis Enables Assessment of Inflammatory and Drug Treatment Responses. International Journal of Molecular Sciences, 19(5): 1314.

Fujii Y, Liu L, Yagasaki L, et al., 2022, Cartilage Homeostasis and Osteoarthritis. International Journal of Molecular Sciences, 23(11): 6316.

Haraden C, Huebner J, Hsueh M, et al., 2019, Synovial Fluid Biomarkers Associated with Osteoarthritis Severity Reflect Macrophage and Neutrophil Related Inflammation. Arthritis Research & Therapy, 21(1): 146.

Tseng S, Reddi A, Cesare P, 2009, Cartilage Oligomeric Matrix Protein (COMP): A Biomarker of Arthritis. Biomarkers Insights, 4: 33–44.

Posey K, Coustry F, Hecht J, 2018, Cartilage Oligomeric Matrix Protein: COMPopathies and Beyond. Matrix Biology, 71–72: 161–173.

Lopez-Franco M, Lopez-Franco O, Murciano-Anton M, et al., 2016, Meniscal Degeneration in Human Knee Osteoarthritis: In Situ Hybridization and Immunohistochemistry Study. Archives of Orthopaedic and Trauma Surgery, 136: 175–183.

Kobayashi M, Kawabata K, Kusaka-Kikushima Aet al., 2016, Cartilage Oligomeric Matrix Protein Increases in Photodamaged Skin. Journal of Investigative Dermatology, 136: 1143–1149.

Schulz J, Nuchel J, Niehoff A, et al., 2016, COMP-Assisted Collagen Secretion—A Novel Intracellular Function Required for Fibrosis. Journal of Cell Science, 129: 706–716.

Schulze K, Nuchel K, Niehof K, et al., 2016, Complex-Assisted Collagen Secretion: An Intracellular Function Required for Fibrosis. Journal of Cell Science, 129(4): 706–716.

Georgiev T, Ivanova M, Kopchev A, et al., 2018, Cartilage Oligomeric Protein, Matrix Metalloproteinase-3, and Coll2-1 as Serum Biomarkers in Knee Osteoarthritis: A Cross-Sectional Study. Rheumatology International, 38: 1–10.

Wanvisa U, Natcha M, Thanyalak T, et al., 2024, Cartilage Oligomeric Matrix Protein (COMP): A Biomarker of Arthritis. Bone & Joint Research, 13(6): 261–271.

Heard B, Martin L, Rattner J, et al., 2012, Matrix Metalloproteinase Protein Expression Profiles Cannot Distinguish Between Normal and Early Osteoarthritic Synovial Fluid. BMC Musculoskeletal Disorders, 23(13): 126.

Runhaar J, Kloppenburg M, Boers M, et al., 2021, Towards Developing Diagnostic Criteria for Early Knee Osteoarthritis: Data from the CHECK Study. Rheumatology, 60: 2448–2455.

Hao H, Zhang J, He Q, et al., 2019, Cartilage Oligomeric Matrix Protein, C-Terminal Cross-Linking Telopeptide of Type II Collagen, and Matrix Metalloproteinase-3 as Biomarkers for Knee and Hip Osteoarthritis (OA) Diagnosis: A Systematic Review and Meta-Analysis. Osteoarthritis and Cartilage, 27: 726–736.

Riegger J, Rehm M, Buchele G, et al., 2020, Serum Cartilage Oligomeric Matrix Protein in Late-Stage Osteoarthritis: Association with Clinical Features, Renal Function, and Cardiovascular Biomarkers. Journal of Clinical Medicine, 9: 268.

Henrotin Y, 2022, Osteoarthritis in Year 2021: Biochemical Markers. Osteoarthritis and Cartilage, 30: 237–248.