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<article xsi:noNamespaceSchemaLocation="http://jats.nlm.nih.gov/publishing/1.1/xsd/JATS-journalpublishing1-mathml3.xsd" dtd-version="1.1" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><front><journal-meta><journal-id journal-id-type="publisher-id">JCNR</journal-id><journal-title-group><journal-title>Journal of Clinical and Nursing Research</journal-title></journal-title-group><issn>2208-3685</issn><eissn>2208-3693</eissn><publisher><publisher-name>Bio-Byword Scientific Publishing Pty. Ltd.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.26689/jcnr.v10i2.14219</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title>Evidence-Based Plan for Environmental Cleaning of Operating Room: Impact on High-Frequency Contact Surfaces</title><url>https://artdesignp.com/journal/JCNR/10/2/10.26689/jcnr.v10i2.14219</url><author>FengLiping,LinKe,HongBinfang,DuanQingluan,WangYuying,FanQi,BaiJing,SunChengxin</author><pub-date pub-type="publication-year"><year>2026</year></pub-date><volume>10</volume><issue>2</issue><history><date date-type="pub"><published-time>2026-03-13</published-time></date></history><abstract>Objective: To develop an evidence-based plan for cleaning operating room and evaluate the impact on high-frequency contact surfaces. Method: The evidence application model of the JBI Evidence-Based Nursing Center was utilized to create a strategy, which was implemented in a tertiary-level hospital in Yunnan Province. The adenosine triphosphate (ATP) biological biofluorescence detection method was used to assess the quality of cleaning before and after the intervention. Results: A total of 17 quality review indicators were established in this study. Following the application of evidence, the implementation rate for 16 quality review indicators increased significantly, from a range of 0–71.6% to 85.3–100%. Moreover, the pass rate of ATP bioluminescence detection on high-frequency contact surfaces increased from 14.07% to 47.10%, with significant difference (p &amp;lt; 0.05). Conclusion: The evidence-based environmental cleaning program proved to enhance the overall cleanliness of operating room and reduce the risk of surgical infections. 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