JCNRJournal of Clinical and Nursing Research2208-36852208-3693Bio-Byword Scientific Publishing Pty. Ltd.10.26689/jcnr.v6i1.2912ArticleResearch and Development of Butterfly Microfluidic Gene Chip for 19 Common Pathogenic Microorganisms of Nosocomial Infection https://artdesignp.com/journal/JCNR/6/1/10.26689/jcnr.v6i1.2912YuJingjing,ZhangXu,TianJinhai,MaRong,HuangQi,CaoJia,WangJia,WangLibin2022612022-01-19Based on butterfly microfluidic gene chip technology, a method for rapid, accurate and efficient detection of 19 common pathogenic microorganisms of nosocomial infection was established, and a butterfly microfluidic gene chip with high-throughput detection was designed and fabricated. Using constant temperature amplification technology, using the polymerase with chain replacement function to react at constant temperature (65?) and combined with microfluidic chip technology, primers were designed according to the target genes of 19 pathogenic microorganisms, and a butterfly microfluidic gene chip which can detect 19 common pathogenic microorganisms of nosocomial infection was made to simplify the inspection operation process and verify the sensitivity of the chip. The butterfly microfluidic gene chip can be used for the rapid and efficient detection of 19 common pathogenic microorganisms of nosocomial infection, and provides a new idea for the detection and auxiliary diagnosis of pathogenic microorganisms of nosocomial infection.

Liu J, 2019, Thoughts on Some Problems of Bloodstream Infection. National Medical Journal of China, (31): 2471-2474.

Chen Y, Ji J, Ying C, et al., 2019, Drug Resistance Monitoring Report of Bloodstream Infection Bacteria in China from 2014 to 2015. Chinese Journal of Clinical Infectious Diseases, 12(1): 24-37.

Leibovici-Weissman Y, Tau N, Yahav D, 2019, Bloodstream Infections in the Elderly: What is the Real Goal?, Aging Clin, DOI: 10.1007/s40520-019-01337-w.

Zhan G, Lu J, Sun J, et al., 2019, Analysis of Clinical Characteristics and Drug Resistance of Bloodstream Infection Caused by Escherichia Coli. Chinese Journal of Infection and Chemotherapy, 19(4): 381-385.

Zhang D, Zheng R, 2019, Preface: Pay Attention to the Progress of Diagnosis and Treatment of Senile Bloodstream Infection. Practical Geriatrics, 33(3): 209-210.

Xie X, Sheng Y, Gao B, et al., 2012, Drug Resistance Analysis and Treatment Strategy of Non-fermenting Bacteria in ICU. Chinese Journal of Nosocomiology, 22(24): 5615-5617.

Li Q, Cai T, Chang Y, 2010, Application of Microarray Technology in the Detection of Clinical Pathogenic Bacteria. Modern Practical Medicine, 22(5): 487-500.