JCNRJournal of Clinical and Nursing Research2208-36852208-3693Bio-Byword Scientific Publishing Pty. Ltd.10.26689/jcnr.v7i4.5085ArticleThe Effect of MMP-9 Inhibitors on the Biological Behavior of Human Oral Squamous Cell Carcinoma SCC15 Cell Line Through PI3K/Akt Signaling Pathwayhttps://artdesignp.com/journal/JCNR/7/4/10.26689/jcnr.v7i4.5085ZhuangZhizheng,HuYan,LiuHongyue2023742023-07-25Objective: To investigate the effect of MMP-9 inhibitor (Mki67) on the biology of human oral squamous cell carcinoma SCC15 cell line and to explore its mechanism of action through PI3K/Akt signaling pathway. Methods: SCC15 cells were extracted, and the supernatant was discarded. The cells were then rinsed twice with PBS, and 0, 2.5, 5, and 10 μL of Mki67 (50 mg/mL) were added to the culture respectively. The inhibition rate of cell proliferation was detected by 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) method, and the cell migration was measured by Transwell chamber test. The cell apoptosis rate was detected by cytometry, and the p-Akt protein content in the cells of each group was determined by a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) kit. Results: The cell proliferation rates of the 2.5 μL, 5 μL, and 10 μL dose groups were all lower than the 0 μL group (P < 0.05) before treatment, and the cell proliferation rates in the 2.5 μL, 5μL, and 10μL dose groups decreased overtime (P < 0.05). After 24 h, with the increase of Mki67 concentration, the number of migration and invasion gradually decreased (P < 0.05), and the number of apoptosis gradually increased (P < 0.05); besides, the relative expression of MMP-9, PI3K, and Akt mRNA decreased gradually (P < 0.05), and the expression level of Akt mRNA was not statistically significant (P > 0.05). Conclusion: MMP-9 inhibitor (Mki67) can inhibit the proliferation and migration of SCC15 cell line and induce apoptosis, and its mechanism of action may be related to the inhibition of PI3K/Akt signaling pathway.

Wang H, Yan B, Li C, 2023, The Molecular Mechanism of Down-Regulating LncRNA ZEB1-AS1 to Promote the Expression of miR-133a-3p Inhibits the Invasion and Migration of Oral Squamous Cell Carcinoma SCC15. Chinese Journal of Gerontology, 43(04): 916–920.

Xu W, 2022, The Effect of PGRN on the Proliferation, Migration, and Invasion of Oral Squamous Cell Carcinoma Cells, dissertation, Shandong University.

Chang B, Xiao R, Zhao H, et al., 2022, Lidocaine Inhibits the Proliferation and Promotes the Apoptosis of Human Oral Cancer Cell Line HN13 Through the PI3K/ Akt Pathway. Chinese Journal of Immunology, 38(10): 1218–1221.

Wang S, Wang Y, Wang Y, 2022, Research Progress of PI3K/AKT Pathway in Oral Squamous Cell Carcinoma. Chinese Medical Innovation, 19(05): 181–184.

Wang M, Park W, Miao W, 2021, Exosome-Mediated LOXL4 Regulates the Expression of Immune Escape-Related Factors in Oral Squamous Cell Carcinoma Through the PI3K/AKT Signaling Pathway. Journal of Immunology, 37(08): 679–685.

Li Y, Chen X, Wei T, 2021, The Effect of MiR-144-3p on the Proliferation, Invasion, Apoptosis and PTEN/PI3K/AKT Signaling Pathway of Oral Squamous Cell Carcinoma Cal27 Cells. Chinese Experimental Diagnostics, 25(02): 250–256.

Wang S, Wang C, 2021, Efficacy of Chemotherapy Combined with PD-1 Inhibitors in the Treatment of NSCLC and its Effect on Serum Levels of Cyfra21-1 and MMP-9. Chongqing Medicine, 50(02): 292–296.

Han Y, Wang Y, 2020, The Effect of MMP-9 Inhibitors on the Biological Behavior of Human Oral Squamous Cell Carcinoma SCC15 Cell Line Through PI3K/ Akt Signaling Pathway. Chinese Journal of Practical Diagnosis and Therapy, 34(08): 805–809.

Liu H, 2020, Overexpression of the Biological Clock Gene Per2 Inhibits the Progression of Oral Squamous Cell Carcinoma by Activating Autophagy Through the PI3K/AKT/ mTOR Pathway, dissertation, Chongqing Medical University.

Wang P, Wei L, Ni G, 2020, The Effect of CCNB1 Gene on the Proliferation, Invasion and Migration of Oral Squamous Cell Carcinoma Cells by Regulating the PI3K/ Akt Signaling Pathway. Journal of Practical Oncology, 34(02): 144–149.

Yan W, 2020, Design of MMP9-Targeted Peptide Inhibitors and Their Anti-Mantle Cell Lymphoma Activity, dissertation, China Medical University.

Da Y, Yang Z, Xia M, et al., 2020, Expression and Significance of Ki-67, PI3K and Beclin1 in Oral Squamous Cell Carcinoma. Shanghai Stomatology, 29(01): 75–79.

Liu L, Cui N, Wang G, 2020, Effect Analysis of MMP-9 Inhibitors on Rat Liver Ischemia-Reperfusion Model. Journal of Clinical and Experimental Medicine, 19(03): 243–246.

Yin P, Su Y, Chen S, et al., 2019, The Effect of MMP-9 Inhibitors on the Proliferation and Invasion of Oral Squamous Cell Carcinoma Cells. Beijing Stomatology, 27(03): 121–125.

Ding H, 2019, Experimental Study on LGALS3BP Regulating the Proliferation and Migration of Oral Squamous Cell Carcinoma Through the PI3K/AKT Pathway, dissertation, Nanjing University.

Yin P, Su Y, Chen S, et al., 2018, Effects of MMP-9 Inhibitors on Proliferation and Invasion of Oral Squamous Cell Carcinoma Cells. Proceedings of the 2018 National Dental Biomedical Academic Annual Conference, 157–158.