JCNRJournal of Clinical and Nursing Research2208-36852208-3693Bio-Byword Scientific Publishing Pty. Ltd.10.26689/jcnr.v9i11.12981ArticleResearch on the Mechanism of the lncRNA DLX6-AS1/miR-26a/PTEN Axis in Regulating the Activation of Hepatic Stellate Cells in Post-hepatitis Liver Fibrosis: An Analysis Based on Systematic Validation and Clinical Translation Methodshttps://artdesignp.com/journal/JCNR/9/11/10.26689/jcnr.v9i11.12981WangYan,GaoChao,FeiDongqin,ZhangFang,ZhangQi20259112025-12-09Objective: To elucidate the role and clinical potential of the lncRNA DLX6-AS1/miR-26a/PTEN axis in liver fibrosis. Methods: Systematic studies were conducted using cellular and animal models through causal validation, bivariate experiments, single-cell sequencing, ROC analysis of clinical samples, and humanized mouse models. Results: LncRNA DLX6-AS1 inhibited PTEN by adsorbing miR-26a, promoting hepatic stellate cell activation in a dose/time-dependent manner; the axis demonstrated excellent diagnostic performance (AUC > 0.9), and its inhibitors effectively reversed fibrosis in vivo. Conclusion: This study provides new biomarkers and targeted therapeutic strategies for liver fibrosis.

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