PARProceedings of Anticancer Research2208-35452208-3553Bio-Byword Scientific Publishing Pty. Ltd.10.26689/par.v9i6.13003ArticleMechanism of Hedysarum Multijugum Maxim. in Treatment of Bladder Cancer Based on Network Pharmacology and Molecular Docking Technologyhttps://artdesignp.com/journal/PAR/9/6/10.26689/par.v9i6.13003TianHong,ZhangYunchen,HuYang,XuanChong Yun,SunLiquan,GuHao2025962025-12-10To investigate the targets and mechanism of Hedysarum Multijugum Maxim (HMM) in treatment of bladder cancer (BC). Based on Traditional Chinese Medicine Systems Pharmacology (TCMSP) and gene databases, active substances and potential targets of HMM were screened, and the HMM-active substances-targets-BC (HATB) regulatory network and PPI network were constructed. Hub targets were screened by Cytoscape. The main active substances and Hub targets were molecularly docked with AutoDock and visualized by PyMOL. 12 Hub targets were screened. Molecular docking showed that active substances mainly acted on MAPK14, MAPK1 and CCND1. The bindings of calycosin to MAPK14, formononetin to MAPK14, and calycosin to CCND1 were stable.

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