SSRScientific and Social Research2661-43322981-9946Bio-Byword Scientific Publishing Pty. Ltd.10.26689/ssr.v7i10.12750ArticleQuantitation Method Research for the Pyrrolizidine Alkaloids (PAs) Determination in Botanicals by HPLC-MS/MShttps://artdesignp.com/journal/SSR/7/10/10.26689/ssr.v7i10.12750LiFan,ZengKe,HuPengfei,LiJinjing,ZhangYanjun,ChangPeter,SwansonGary20257102025-10-29A HPLC-MS/MS method for the determination of Pyrrolizidine alkaloids (PAs) in green tea, orange pekoe tea, hibiscus flower, chamomile flower, and cardamom seed was developed and validated. Its precision, accuracy, linearity, specificity, LOQ, LOD, and ruggedness were validated following AOAC Guidance for Single Laboratory Validation Procedure. The recovery of the analytes is in a range of 70%–130%. LOQ for 21 PAs in the above botanicals is in a range of 0.8 to 6.5 ppb.

Rauter AP, Palma FB, Justino J, et al., 2002, Natural Products in the New Millennium: Prospects and Industrial Application (Proceedings of the Phytochemical Society of Europe, Vol. 47). Springer, Dordrecht.

Wang J, Wang CH, Wang ZT, 2007, Advancement of Investigation on Cytotoxicity and Mechanism of Pyrrolizidine Alkaloids. International Journal of Pharmaceutical Research, 34(4): 246–249 + 258.

Peter P, Fu QS, 2004, Pyrrolizidine Alkaloids—Genotoxicity, Metabolism Enzymes, Metabolic Activation, and Mechanisms. Drug Metabolism Reviews, 2004(36): 1–55.

Wiedenfeld H, 2011, Plants Containing Pyrrolizidine Alkaloids: Toxicity and Problems. Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment, 28(3): 282–292.

Jia N, Zeng SD, Chen WH, 2022, Determination of Pyrrolizidine Alkaloids in Dendrobium officinale Kimura et Migo by UPLC-MS/MS. Modern Food Science & Technology, 38(2): 295–304.

Zhang F, Wang CH, Wang ZT, 2006, Analysis of Pyrrolizidine Alkaloids in Plants. Natural Product Research and Development, 18(6): 1057–1063.

Wu Y, Liu BL, 2020, Safety Considerations of Traditional Chinese Medicine Containing Pyrrolizidine Alkaloids. The Chinese Journal of Clinical Pharmacology, 36(23): 3954–3956

Han HL, Jiang CL, 2021, Pyrrolizidine Alkaloids in Tea: A Review of Analytical Methods, Contamination Levels and Health Risk. Food Science, 42(17): 255–266.

Zhu L, Hua RM, Wang LY, 2021, Research Progress on Detection and Analysis Methods of Pyrrolizidine Alkaloids in Plants. Quality and Safety of Agro-Products, 2021(4): 36–42 + 46.

Han HL, Jiang CL, 2021, Determination of 15 Pyrrolizidine Organisms in Tea Beverage by UPLC-MS/MS. Quality and Safety of Agro-Products, 2021(4): 18–22 + 29.

Guo WH, Zhou JH, Huang JP, et al., 2014, Determination of Alkaloids in Honey by Dispersive Solid Phase Extraction and High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry. 2014, 42(10). 1453-1458.

Liu PY, Zhang H, Zhang GR, et al., 2019, Determination of 20 kinds of Pesticides Residues in Fruits and Vegetables by Microwave–assisted Extraction-dispersive Solid-phase Extraction-high Performance Liquid Chromatography-tandem Mass Spectrometry. Journal of Food Safety & Quality, 10(21): 7379–7386.

Huang DY, Wang Y, Li Q, et al., 2018, Pyrrolizidine Alkaloids and its Source Analysis in Tea. Journal of Food Safety and Quality, 9(2): 230–236.

Federal Institute for Risk Assessment (BfR), 2014, Method Protocol BFR-PA-Tea-2.0/2014: Determination of Pyrrolizidine Alkaloids (PA) in Plant Material by SPE-LC-MS/MS. BfR, Berlin.

Federal Institute for Risk Assessment (BfR), 2013, BfR Opinion No. 018/2013: Pyrrolizidine Alkaloids in Herbal Teas and Teas. BfR, Berlin.

Federal Institute for Risk Assessment (BfR), 2013, Method Protocol BFR-PA-Honey-1.0/2013: Determination of Pyrrolizidine Alkaloids (PA) in Honey by SPE-LC-MS/MS. BfR, Berlin.

Agilent Technologies, 2016, Quantitation of Pyrrolizidine Alkaloids in Honey and Herbal Teas by UHPLC/MS/MS (Application Note No. 5991-7301EN). Agilent Technologies, Santa Clara.