SSRScientific and Social Research2661-43322981-9946Bio-Byword Scientific Publishing Pty. Ltd.10.26689/ssr.v8i3.14464ArticleMetagenomic Analysis of Rhizosphere Microbial Community Structure in Tea-Fruit Intercropping Systemshttps://artdesignp.com/journal/SSR/8/3/10.26689/ssr.v8i3.14464LiMingyan,YanKuan,LiuJianghua2026832026-04-15In the tea fruit intercropping system, intercropping between tea trees and fruit trees can enhance plant nutrient utilization advantages, and plant growth is closely related to the rhizosphere microorganisms of plants. In order to explore the community structure characteristics of rhizosphere soil microorganisms under tea fruit intercropping mode, Method: This study used the Illumina Novaseq sequencing platform to perform metagenomic sequencing on three types of rhizosphere soil, with tea tree monoculture and loquat tree monoculture as controls, to analyze the differences in rhizosphere microbial community structure between tea fruit intercropping. The NR annotation results showed that under the tea fruit intercropping mode, the abundance of the dominant phylum Actinobacteria showed a decreasing trend, while the abundance of Acidobacteria and Proteobacteria increased. The relative abundance of unclassified Acidobacteria, unclassified Actinobacteria of the dominant phylum Actinobacteria, and unclassified Actinobacteria of the phylum Actinobacteria all increased. The annotation results of COG functional genes showed that in the functional composition of rhizosphere soil microorganisms in tea fruit intercropping, functions related to amino acid transport metabolism occupy a dominant position, and compared with tea monoculture, the relative abundance of functions related to amino acid transport metabolism, energy generation, and conversion is significantly increased. The annotation results of KEGG functional genes showed that pathways related to metabolism occupy a dominant position in the functional composition of the three rhizosphere microorganisms, and compared with tea monoculture, the abundance of functions related to energy metabolism was significantly increased in tea fruit intercropping.  After intercropping tea and fruit, their nitrogen fixation and disease resistance abilities were increased, soil fertility was improved, and energy metabolism was enhanced, significantly improving nitrogen metabolism and enhancing the growth status of tea tree roots.

Parolin P, Bresch C, Desneux N, et al., 2012, Secondary Plants Used in Biological Control: A Review. International Journal of Pest Management, 58(2): 91–100.

Cao QM, Wang YP, Qin JW, et al., 2020, Effects of Compound Planting on Soil Properties and Tea Chemical Quality in Hainan Tea Gardens. Southwest China Journal of Agricultural Sciences, 33(7): 1504–1509.

Handelsman J, Rondon MR, Brady SF, et al., 1998, Molecular Biological Access to the Chemistry of Unknown Soil Microbes: A New Frontier for Natural Products. Chemical Biology, 5(10): R245–R249.

Liu LY, Cui HF, Tian G, 2013, Application of High-throughput Sequencing Technology in Metagenomics. Chinese Medicinal Biotechnology, 8(3): 196–200.

Lin XY, Li AS, Luo T, et al., 2024, Study on Fruit-tea Intercropping Cultivation Patterns. Anhui Agricultural Science Bulletin, 30(5): 21–25.

Wen B, Zhang XL, Ren S, et al., 2019, Characteristics of Soil Nutrients, Heavy Metals and Tea Quality in Different Intercropping Patterns. Agroforestry Systems, 94(3): 963–974.

Wang H, 2015, Responses of Soil Microbial Community Structure to Tea Planting Age, Fertilization and High Temperature Conditions in Tea Gardens, thesis, Zhejiang University.

Hu MY, Li YY, Ge CR, et al., 2021, Research Progress and Application Prospects of Associative Nitrogen Fixation in Gramineous Plants. Chinese Journal of Eco-Agriculture, 29(11): 1815–1826.

Du X, 2021, Microbial Community Structure and Functional Potential of Rhizosphere Soil of Plants in the Coastal Zone of Daihai Lake, thesis, School of Architecture and Civil Engineering, Inner Mongolia University of Science and Technology.

Dong MH, Gu JR, Liu TF, et al., 2015, Comparative Analysis of Soil Nutrients in Different Tea-fruit Intercropping Tea Gardens in Dongting Mountain, Suzhou. China Tea, 37(5): 19–20.

Li QS, Lei WX, Liu JX, et al., 2021, Effects of Ophiopogon japonicus Intercropping on Soil Physical and Chemical Properties and Microbial Community Structure in Tea Gardens. Journal of Southern Agriculture, 52(12): 3366–3374.

Tian YH, Liang YF, Wang GH, et al., 2001, Study on Ecological Effects of Artificial Ecological Tea Gardens. Journal of Tea Science, 21(2): 170–174.

Wang WX, Li XW, Huang WG, et al., 2020, Correlation between Bacterial Community Composition and Diversity of Rhizosphere Soil of Ammopiptanthus mongolicus and Ecological Factors. Acta Ecologica Sinica, 40(23): 8660–8671.

Fierer N, Ladau J, Clemente JC, et al., 2013, Reconstructing the Microbial Diversity and Function of Pre-agricultural Tallgrass Prairie Soils in the United States. Science, 342(6158): 621–624.

Navarrete AA, Soares T, Rossetto R, et al., 2015, Verrucomicrobial Community Structure and Abundance as Indicators for Changes in Chemical Factors Linked to Soil Fertility. Antonie Van Leeuwenhoek, 108(3): 741–752.